Prostaglandin induction of icer expression in osteoblastic cells and mouse calvariae
Digital Document
Handle |
Handle
http://hdl.handle.net/11134/20004:20201190
|
||||||
---|---|---|---|---|---|---|---|
Persons |
Persons
Creator (cre): Ammari, Zahra
Major Advisor (mja): Kream, Barbara E.
Associate Advisor (asa): Pilbeam, Carol
Associate Advisor (asa): Nanda, Ravindra
|
||||||
Title |
Title
Title
Prostaglandin induction of icer expression in osteoblastic cells and mouse calvariae
|
||||||
Origin Information |
Origin Information
|
||||||
Parent Item |
Parent Item
|
||||||
Resource Type |
Resource Type
|
||||||
Digital Origin |
Digital Origin
born digital
|
||||||
Description |
Description
Inducible cAMP early repressor (ICER) is an early response gene that is transcribed from an intronic promoter of the CREM gene. ICER and other CREM transcripts are expressed in pineal gland, pituitary gland, sertoli cells, as well as bone and are thought to have a role in the physiology of circadian rhythms and spermatogenesis. ICER proteins consist of the DNA binding domains of CREM and act as dominant negative repressors of gene transcription. Parathyroid hormone induces ICER expression in osteoblastic cells through the cAMP/PKA pathway. Prostaglandins also work through the cAMP/PKA pathway and have a myriad of effects on bone metabolism. To determine whether prostaglandins induce ICER expression in osteoblastic cells, MC3T3-E1 cells and mouse calvariae were treated with 1 gM PGE2, 1 gM PGF2a and 10 nM PTH. The RNA extracted from these osteoblastic cells and calvariae was analysed for ICER and osteocalcin expression using RT-PCR and/or Northern blot analysis. PGE and PGF both induced ICER expression, but PGFwas a weaker agonist. This induction was maximal at 10 gM PGE2 and 1 gM PGF. and returned to basal levels by 0.1 nM. ICER expression was also time dependent with a peak at 2 h that returned to baseline by 10 h. We also studied the relation between cell differentiation and the expression of ICER by treating MC3T3-E1 cells with or without ascorbic acid for up to three weeks. Ascorbic acid was not absolutely necessary for the differentiation of MC3T3-E1 osteoblastic cells. However, induction of ICER increased as MC3T3-E1 cells differentiated with ascorbic acid. We conclude that protaglandins Ez and Finduce ICER expression in MC3T3-E1 osteoblastic cells and neonatal mouse calvariae and this induction is greater as the cells differentiate. ICER may play a role in prostaglandin mediated gene expression in bone.
|
||||||
Genre |
Genre
|
||||||
Organizations |
Organizations
Degree granting institution (dgg): University of Connecticut
|
||||||
Held By | |||||||
Use and Reproduction |
Use and Reproduction
These Materials are provided for educational and research purposes only.
|
||||||
Degree Name |
Degree Name
Master of Dental Science
|
||||||
Degree Level |
Degree Level
Master
|
||||||
Degree Discipline |
Degree Discipline
Dental Science
|
||||||
Local Identifier |
Local Identifier
OC_SODM_th_014
45357130
ASC Thesis 12600
|