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http://hdl.handle.net/11134/20002:860644745
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Description
Bone tissue engineering has been proposed as a more effective and efficient alternative option for bone repair and regeneration. Here, we propose a two-pronged approach for enhance scaffold-guided bone regeneration. Second to developing optimized PLGA optimally-porous scaffolds, we will pre-vascularize our constructs in vitro in order to reduce vascularization time, and enhance bone formation in vivo. We will pre-vascularize our constructs by seeding and culturing them with a combination of two cell populations required for angiogenesis and osteogenesis: peripheral blood derived -endothelial progenitor cells (EPCs) and bone marrow derived -mesenchymal stem cells (MSCs). Previous studies have demonstrated EPCs and MSCs promote enhanced bone regeneration via the stimulation of neo-vascularization. We will systematically examine of the combination of these two required cell populations and the optimally-porous PLGA scaffolds, and the resultant effects on healing critically sized segmental bone defects. We hypothesize that our pre-vascularized, optimally-porous PLGA scaffolds will substantially improve the performance of PLGA microsphere scaffolds by promoting angiogenesis, and significantly enhancing bone formation in vivo.
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Use and Reproduction
These materials are provided for educational and research purposes only.
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